Fig. 2

IL-6R protein expression in human retinal endothelial cells. A-C Representative flow cytometry plots from one cell isolate: (A) Debris and doublets were excluded based on forward scatter (FSC) and side scatter (SSC) properties. B CD31-positive were gated in each sample relative to unstained controls. C IL-6R expression was assessed in non-permeabilized and permeabilized CD31-positive cells. Blue and red traces indicate the fluorescence in unstained and IL-6R antibody-stained of CD31-positive cells, respectively. D Histograms showing percentage of CD31-positive cells expressing IL-6R for individual primary cell isolates (n = 5 donors) and the expanded cell isolate (n = 4 experiments). Bars indicate mean. Non-permeabilized and permeabilized groups were compared by donor-paired (primary cell isolates) and experiment-paired (expanded cell isolate) 2-tailed Student’s t-test: p > 0.05