Table 2 Acanthamoeba castellanii cyst viability counts after exposure to different MPS formulations. Cultures containing A. castellanii cysts were co-mixed for varying contact times, stained with Trypan blue, and then transferred to a hemocytometer for microscopic enumeration. A total of nine grids were counted; separate experiments were performed and quantitative analysis performed by two technicians blinded to the treatments. Counts reflect percentage dead cysts of total counted within the collective nine grids; numbers expressed as arithmetic mean ± standard deviation
MPS | Contact time (min) | |||
|---|---|---|---|---|
15 | 30 | 60 | 90 | |
PBS (sham) | – | – | – | – |
ASP-57 | 72.8 ± 26.9 | 96.4 ± 5.1 | 97.5 ± 3.5 | 100 ± 0.0 |
BioTrue | 5.6 ± 2.9 | 15.6 ± 22.1 | 24.2 ± 22.4 | 32.9 ± 4.9 |
Opti Free | 23.2 ± 5.8 | 23.6 ± 2.0 | 19.9 ± 15.3 | 51.1 ± 7.9 |