Fig. 2
From: Ocular mycobacteriosis—dual infection of M. tuberculosis complex with M. fortuitum and M. bovis
a Formation of moist colonies on LJ medium seen within 3 days of incubation. b Tiny pink colonies formed by the rapid growers on Mac Conkey agar. c Presence of M. fortuitum confirmed by using PCR targeting specific primers for M. fortuitum. L1—100 bp molecular marker, L2—275 bp positive control of M. fortuitum (black arrow), L3 and L4—DNA extracted from culture and positive for M. fortiuitum, L5 and L6—DNA extracted from patient VF sample positive for M. fortuitum, L7—negative control. Presence of M. fortuitum confirmed by using PCR targeting specific primers for M. fortuitum (black arrow). L1—100 bp MM, L2—positive control (black arrow), L3 and L4—DNA extracted from culture isolate from VF sample of patient, L5—DNA extracted from VF sample of patient, L6—negative control. d Presence of M. bovis confirmed by using PCR targeting the specific Hup B genes. L1—100 bp MM, L2—positive control for M. bovis (black arrow), L3 and L4—DNA extracted from culture of M. tuberculosis complex, L5—DNA extracted from VF of patient sample, L6—negative control