Figure 2
From: OX40 ligand expression abrogates the immunosuppressive function of retinal pigment epithelium
Time trial after vector transfection. (a) Bar graph representing the expression pattern of the fluorescent protein (YFP) and OX40L after transfection of eYFP-C1-OX40L into RPE at five time points (time point 1 = 30 min, time point 2 = 1 h, time point 3 = 4 h, time point 4 = 6 h, and time point 5 = 24 h). Time-dependent increased expression is observed. (b) Flow cytometric analysis at 4 h after transfection of RPE cells with eYFP-C1-OX40L, with YFP expression (bold) in the left panel and OX40L expression (bold) in the right panel (the thinner lines represent RPE that was not transfected). (c) Bar graph expression pattern of YFP and OX40L after transfection of eYFP-N1-OX40L into RPE cells at the same time points. (d) Flow cytometric analysis at 4 h after transfection of RPE cells with eYFP-N1-OX40L.