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Table 4 Conjugal transfer of antibiotic resistance and plasmids to E. coli DH5α by some of the selected bacterial isolates recovered from patients with conjunctivitis

From: Bacteriologic and plasmid analysis of etiologic agents of conjunctivitis in Lagos, Nigeria

Donor strain

Antibiotype

Plasmid profile

Antibiotype transferred to E. coli DH5α

R plasmid transferred to E. coli DH5α

Conjugation frequencya

P. aeruginosa NG-02

Chl Str Amp Gen

21.3, 5.0, 1.2, 1.0

Chl Str Amp

21.3, 5.0

1.8 × 10–7

P. aeruginosa NG-07

Amo Amp Ctx Ofl

21.3

Amp

–

1.4 × 10–5

E. coli NG-05

Chl Amp Str Amk

21.3, 5.0

Amp Str

21.3

1.9 × 10–7

E. coli NG-01

Chl Amp Gen Amo

15.2, 10.0, 4.1

Chl Amp

15.2

1.8 × 10–6

Klebsiella oxytoca NG-03

Chl Str

4.1, 2.7

Chl

–

2.1 × 10–7

S. aureus NG-18

Amp Str Amo

15.2, 9.0, 5.0, 2.0

Str

–

1.9 × 10–5

S. epidermidis NG-22

Chl Amp Str

15.2, 2.0

–

–

ND

  1. ND not determined, Chl chloramphenicol, Amp ampicillin, Str streptomycin, Amo amoxicillin, Gen gentamicin, Amk amikacin, Ofl ofloxacin, Ctx cefotaxime
  2. aConjugation frequency is defined as the number of transconjugants as a proportion of total bacterial count in the nonselective medium