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Table 2 Fungal DNA detection and identification to diagnose mycotic keratitis

From: Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience

First author Year No. of eyes Target DNA Detection Molecular identification Level of molecular identification
Alexandrakis16 1998 3 Cutinase PCR Specific PCR Fusarium solani
Ferrer8 2001 11 ITSs-5.8S rRNA Nested PCR DNA sequencing Species level
Gaudio14 2002 30 18S rRNA Nested PCR Nested PCR C. albicans, A. fumigatus, F. oxysporum
Ferrer21 2002 1 ITSs-5.8S rRNA PCR DNA sequencing Alternaria alternata
Rishi22 2003 1 ITSs-5.8S rRNA PCR Not done Not done
Guarro23 2003 1 ITSs-5.8S rRNA Culture DNA sequencing Fusarium polyphialidicum
Ferrer24 2003 1 ITSs-5.8S rRNA PCR DNA sequencing Alternaria infectoria
Mancini25 2005 1 ITSs-5.8S rRNA PCR DNA sequencing Scedosporium apiospermum
Kumar17 2005 4 ITSs-5.8S rRNA PCR SSCP Genus level
Kumar18 2005 4 ITSs-5.8S rRNA PCR SSCP Genus level
Kumar19 2006 4 28S rRNA PCR SSCP Genus level
Ferrer9 2006 1 ITSs-5.8S rRNA PCR DNA sequencing Candida parapsilosis
Donnio26 2006 1 ITSs-5.8S rRNA PCR DNA sequencing Lasiodiplodia theobromae
Ghosh27 2007 32 ITSs-5.8S rRNA PCR DNA sequencing Species level
Suzuki28 2007 1 ITSs-5.8S rRNA PCR DNA sequencing Malasezia restricta
Yoon29 2008 1 18S rRNA PCR DNA sequencing Scedosporium apiospermum
Laich30 2008 1 ITSs-5.8S and 28S rRNA PCR DNA sequencing Alternaria alternata
Kim31 2008 108 18S rRNA PCR DNA sequencing Species level
Embong32 2008 30 18S rRNA PCR DNA sequencing Species level
Bagyalakshmi33 2008 12 ITSs-5.8S rRNA PCR DNA sequencing Botryosphaeria Lasiodiplodia ssp., Thielavia tortuosa, Glomerulla singulata, Macrophomina phaseolina, Rhizoctonia bataticola, and Podospora spp.
Vengayil12 2009 40 28S rRNA PCR not done Not done
Dong34 2009 42 ITSs-5.8S rRNA Microarray Fluorescent beads F. solani, F. moniliforme, F. oxysporum, A. fumigatus, A. flavus
Badiee35 2009 38 18S rRNA Nested PCR Nested PCR C. albicans, A. fumigatus, F. solani
Ferrer10, 11 2010 1 ITSs-5.8S rRNA PCR DNA sequencing Pyrenochaeta keratinophila
Itahashi36 2010 40 ITS2 Real-time PCR Real-time PCR Candida spp.
Menassa 37 2010 15 ITSs-5.8S rRNA Real-time PCR Real-time PCR C. albicans, Aspergillus spp., Fusarium spp.