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Table 1 Sample and laboratory diagnosis of fungal keratitis

From: Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience

Patient Sample Date Stain Culture Identification culture Fungal PCR Molecular identification Topical/oral treatment
1 Corneal scrape 15/01/2000 Septate hyphae Positive (2 days) Aspergillus fumigatus Positive Aspergillus fumigatus Amphotericin B/fluconazole
2 Corneal scrape 06/10/2000 Negative Negative   Positive Aspergillus niger Amphotericin B
3 Corneal scrape 12/02/2001 Negative Positive (7 days) Alternaria alternata Positive Alternaria alternata Amphotericin B/fluconazole
4 Corneal scrape 15/06/2001 Hyphae Positive (6 days) Scedosporium apiospermum Positive Scedosporium apiospermum Natamicin/voriconazole
5 Corneal scrape 19/03/2002 Yeast Positive (1 day) Candida albicans Positive Candida albicans Amphotericin B/fluconazole
6 Corneal scrape 02/08/2002 Hyphae Positive (7 days) Alternaria sp. Positive Alternaria infectoria Amphotericin B/fluconazole
7 Corneal scrape 18/11/2002 Broken hyphae Negative   Positive Aspergillus oryzae  
8 Corneal scrape 18/02/2003 Septate hyphae and conidia Positive (1 day) Fusarium sp. Positive Fusarium oxysporum Amphotericin B/econazole
9 Corneal biopsy 03/08/2003 Negative Negative   Positive Candida parapsilosis  
  Corneal biopsy 10/08/2003 Yeast Positive (2 days) Candida parapsilosis Positive Candida parapsilosis Amphotericin B/voriconazole
10 Corneal scrape 07/07/2004 Septate hyphae Positive (1 day) Aspergillus niger Positive Aspergillus niger Amphotericin B
11 Corneal scrape 20/05/2005 Negative Negative   Positive Candida famata Amphotericin B/itraconazole
12 Corneal scrape 03/08/2005 Yeast Negative   Positive Candida parapsilosis Amphotericin B/voriconazole
13 Corneal scrape 13/10/2005 Negative Negative   Negative   
  Corneal scrape 20/10/2005 Yeast and hyphae Negative   Positive Candida sake Natamicin/itraconzaole
14 Corneal scrape 02/02/2007 Negative Negative   Negative   
  Corneal scrape 02/02/2007 Negative Negative   Positive Fusarium solani  
  Corneal biopsy 07/02/2007 Hyphae Positive (7 days) Fusarium solani Positive Fusarium solani Voriconazol/voriconazole
15 Corneal scrape 05/03/2007 Septate hyphae Negative   Positive Pyrenochaeta keratinophila  
  Corneal biopsy 13/03/2007 Septate and branched hyphae Positive (6 days) Unidentified fungi Positive Pyrenochaeta keratinophila Natamicin/itraconazole
16 Corneal scrape 07/04/2008 Septate hyphae Positive (2 days) Sterile mycelium Positive Paecilomyces sp.  
  Corneal scrape 03/06/2008 Septate hyphae Negative   Positive Paecilomyces sp.  
  Cornea 06/06/2008 Septate and branched hyphae Positive (35 days) Sterile mycelium Positive Paecilomyces sp. Natamicin/itraconazole
17 Corneal scrape 19/08/2008 Negative Positive (6 days) Unidentified fungi Positive Scedosporium apiospermum Voriconazole/voriconazole
18 Corneal scrape 04/02/2009 Yeast Positive (1 day) Candida sp. Positive Candida dublinensis Fluconazole/fluconazole
19 Corneal biopsy 05/06/2009 Septate and branched hyphae Positive (2 days) Fusarium solani Positive Fusarium solani Natamicin/voriconazole
20 Corneal scrape 23/06/2009 Negative Positive (2 days) Candida sp. Positive Candida parapsilosis Fluconazole/fluconazole