Table 1 Sample and laboratory diagnosis of fungal keratitis
From: Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience
Patient | Sample | Date | Stain | Culture | Identification culture | Fungal PCR | Molecular identification | Topical/oral treatment |
|---|---|---|---|---|---|---|---|---|
1 | Corneal scrape | 15/01/2000 | Septate hyphae | Positive (2 days) | Aspergillus fumigatus | Positive | Aspergillus fumigatus | Amphotericin B/fluconazole |
2 | Corneal scrape | 06/10/2000 | Negative | Negative | Positive | Aspergillus niger | Amphotericin B | |
3 | Corneal scrape | 12/02/2001 | Negative | Positive (7 days) | Alternaria alternata | Positive | Alternaria alternata | Amphotericin B/fluconazole |
4 | Corneal scrape | 15/06/2001 | Hyphae | Positive (6 days) | Scedosporium apiospermum | Positive | Scedosporium apiospermum | Natamicin/voriconazole |
5 | Corneal scrape | 19/03/2002 | Yeast | Positive (1 day) | Candida albicans | Positive | Candida albicans | Amphotericin B/fluconazole |
6 | Corneal scrape | 02/08/2002 | Hyphae | Positive (7 days) | Alternaria sp. | Positive | Alternaria infectoria | Amphotericin B/fluconazole |
7 | Corneal scrape | 18/11/2002 | Broken hyphae | Negative | Positive | Aspergillus oryzae | ||
8 | Corneal scrape | 18/02/2003 | Septate hyphae and conidia | Positive (1 day) | Fusarium sp. | Positive | Fusarium oxysporum | Amphotericin B/econazole |
9 | Corneal biopsy | 03/08/2003 | Negative | Negative | Positive | Candida parapsilosis | ||
Corneal biopsy | 10/08/2003 | Yeast | Positive (2 days) | Candida parapsilosis | Positive | Candida parapsilosis | Amphotericin B/voriconazole | |
10 | Corneal scrape | 07/07/2004 | Septate hyphae | Positive (1 day) | Aspergillus niger | Positive | Aspergillus niger | Amphotericin B |
11 | Corneal scrape | 20/05/2005 | Negative | Negative | Positive | Candida famata | Amphotericin B/itraconazole | |
12 | Corneal scrape | 03/08/2005 | Yeast | Negative | Positive | Candida parapsilosis | Amphotericin B/voriconazole | |
13 | Corneal scrape | 13/10/2005 | Negative | Negative | Negative | |||
Corneal scrape | 20/10/2005 | Yeast and hyphae | Negative | Positive | Candida sake | Natamicin/itraconzaole | ||
14 | Corneal scrape | 02/02/2007 | Negative | Negative | Negative | |||
Corneal scrape | 02/02/2007 | Negative | Negative | Positive | Fusarium solani | |||
Corneal biopsy | 07/02/2007 | Hyphae | Positive (7 days) | Fusarium solani | Positive | Fusarium solani | Voriconazol/voriconazole | |
15 | Corneal scrape | 05/03/2007 | Septate hyphae | Negative | Positive | Pyrenochaeta keratinophila | ||
Corneal biopsy | 13/03/2007 | Septate and branched hyphae | Positive (6 days) | Unidentified fungi | Positive | Pyrenochaeta keratinophila | Natamicin/itraconazole | |
16 | Corneal scrape | 07/04/2008 | Septate hyphae | Positive (2 days) | Sterile mycelium | Positive | Paecilomyces sp. | |
Corneal scrape | 03/06/2008 | Septate hyphae | Negative | Positive | Paecilomyces sp. | |||
Cornea | 06/06/2008 | Septate and branched hyphae | Positive (35 days) | Sterile mycelium | Positive | Paecilomyces sp. | Natamicin/itraconazole | |
17 | Corneal scrape | 19/08/2008 | Negative | Positive (6 days) | Unidentified fungi | Positive | Scedosporium apiospermum | Voriconazole/voriconazole |
18 | Corneal scrape | 04/02/2009 | Yeast | Positive (1 day) | Candida sp. | Positive | Candida dublinensis | Fluconazole/fluconazole |
19 | Corneal biopsy | 05/06/2009 | Septate and branched hyphae | Positive (2 days) | Fusarium solani | Positive | Fusarium solani | Natamicin/voriconazole |
20 | Corneal scrape | 23/06/2009 | Negative | Positive (2 days) | Candida sp. | Positive | Candida parapsilosis | Fluconazole/fluconazole |