Fig. 4

Immunofluorescence colocalization of nitrotyrosine with apoptotic cells in retinas of patients with sympathetic ophthalmia (SO). SO globes were labeled using polyclonal rabbit antinitrotyrosine (primary antibody)and sheep-antirabbit IgG conjugated with flourescein (secondary antibody), followed by TUNEL staining using a TACS TdT Apoptosis Detection Kit (American Qualex). Nitrotyrosine was localized primarily in the photoreceptor inner segments (IS). Apoptotic cells were detected mainly in the outer nuclear layer (ONL), with a few apoptotic cells in the inner nuclear layer (INL) in SO retinas