Fig. 3

Immunofluorescence localization of 8-OHdG in the retinas of wild-type (WT; C57BL/6) mice and TLR4-deficient (TLR4^−/−) mice with EAU day 7 after immunization. Tissues were labeled using the polyclonal antibody against 8-OHdG and Texas Red dye-conjugated donkey anti-goat IgG as the primary and secondary antibodies respectively. No 8-OHdG staining was present in the nonimmunized (control) WT and TLR4^−/− mice. In the WT mice with EAU, intense 8-OHdG staining was detected in the inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), and in the inner segments of the photoreceptor layer; such staining was significantly reduced in the outer plexiform layer and inner segments and was absent in the remainder of the retina in the TLR4^−/− mice during early EAU