A diagnostic dilemma: infectious versus noninfectious multifocal choroiditis with panuveitis
© Vemulakonda et al.; licensee Springer. 2013
Received: 10 September 2012
Accepted: 12 September 2012
Published: 28 January 2013
The objective of this study was to report a diagnostic dilemma in a patient with multifocal choroiditis. This is a case report study.
A 68-year-old female presented with new onset of floaters in both eyes and diagnosed with bilateral panuveitis. Her visual acuity was 20/200 in both eyes. Slit-lamp examination showed 1+ anterior chamber cells in both eyes. Ophthalmoscopic examination of both eyes showed vitreous cells, optic disc edema, small amounts of subretinal hemorrhage, and punctate choroidal lesions throughout the fundus. Laboratory work-up revealed a positive QuantiFERON-TB Gold result, and the patient was started on antituberculosis medications. However, given the patient’s intolerance to antituberculosis medications and progressive worsening of vision, she underwent a chorioretinal biopsy to assist with determining a definitive diagnosis. Biopsy results showed noncaseating granulomas and were negative for an infectious etiology. The patient was diagnosed with ocular sarcoidosis and started on immunomodulatory therapy for sarcoid-related multifocal choroiditis.
Multifocal chorioretinal lesions of unknown etiology can present as a diagnostic and therapeutic dilemma. Laboratory work-up is useful in determining an etiology; however, more invasive procedures, such as chorioretinal biopsy, may be necessary to guide treatment.
KeywordsMultifocal choroiditis Sarcoidosis Mycobacterium tuberculosis Granulomatous inflammation
A 68-year-old nurse presented with new onset of floaters in both eyes in August 2009. She noted her symptoms to be painless without eye redness or discharge. She stated that her symptoms progressively worsened with decreasing visual acuity. She was initially seen by an ophthalmologist 2 months after her symptoms started and was diagnosed with bilateral panuveitis. She denied any trauma or injury to the eye, or having any previous ocular history. Her past medical history was significant for insulin-dependent type 2 diabetes mellitus, hypertension, and hypercholesterolemia. She has a history of being purified protein derivative (PPD) positive with a negative chest X-ray, for which she had never received treatment.
Laboratory investigations revealed a white blood cell count of 11.7 × 109/L. Her erythrocyte sedimentation rate, angiotensin-converting enzyme, liver function test, serum urea and electrolytes, and chest X-ray were normal. Treponema pallidum particle agglutination assay was nonreactive, and Bartonella titers were negative. She was positive for QuantiFERON-TB Gold, but had a negative chest CT scan and normal brain MRI scan.
After consultation with an infectious disease specialist, she was started on prednisone and antituberculosis medications to cover a possible infection given her positive QuantiFERON-TB Gold test result and prior positive PPD test. The patient developed severe nausea after starting her antituberculosis medications and immediately stopped taking these medications. By this time, her vision had decreased to between 6/200 and 20/500 in the right eye but remained 20/200 in the left eye.
Given the need for immunosuppression along with the patient’s intolerance to antituberculosis medications, a definitive diagnosis for the cause of her choroiditis was urgently needed. As a result, she underwent a pars plana vitrectomy and chorioretinal biopsy of the right eye to confirm whether the etiology of the patient’s multifocal choroiditis was related to a possible extrapulmonary Mycobacterium tuberculosis infection.
Chorioretinal results effectively ruled out an infectious cause for her uveitis. Following this negative biopsy, the patient was diagnosed with ocular sarcoidosis and was started on immunomodulatory therapy for sarcoid-related multifocal choroiditis. She was placed on long-term methotrexate and had resolution of her disc edema, inflammation, and retinal hemorrhages by 1-year follow-up, and stabilization of her chorioretinal lesions. Unfortunately, her visual acuity remained poor given the chronicity of her disease and continued to decline in her right eye secondary to cataract progression.
Patients presenting with multifocal choroiditis and papillitis can be a diagnostic dilemma. Important diagnoses to consider in the differential when managing these patients include tuberculosis, sarcoidosis, histoplasmosis, syphilis, posterior scleritis, intraocular lymphoma, diffuse unilateral subacute neuroretinitis, and Lyme disease. In addition, the various white dot syndromes should also be considered. An initial laboratory work-up should be performed in all patients.
However, even in the setting of positive laboratory results, the diagnosis may still prove to be difficult. Our patient is a nurse and therefore at increased risk for infectious exposure due to her occupational setting. She had a known history of testing positive for purified protein derivative and also had a positive QuantiFERON-TB Gold test result, which would suggest a mycobacterial infectious etiology. Unlike the pulmonary disease, the diagnosis and treatment of ocular tuberculosis is challenging in the absence of clinically apparent pulmonary disease. The diagnosis is complicated by difficulties obtaining ocular tissue, difficulties isolating the organisms from aqueous aspirates, tests that are neither sensitive nor specific, and the variability in clinical presentation . In an analysis by Rosenbaum and Wernick  using Bayes’ theorem to analyze the utility of purified protein derivative testing, they calculated that a patient with uveitis and a positive purified protein derivative test result has a 1% likelihood of having tuberculosis. This low probability would suggest low utility in ordering a PPD in routine evaluation of patients with uveitis. Newer testing modalities with interferon-gamma release assays (QuantiFERON-TB Gold or In-Tube and T-SPOT TB) have improved the specificity of the diagnosis as they are more specific markers of M. tuberculosis infection and previous exposure and are not influenced by BCG vaccination or exposure to atypical mycobacteria. However, these testing modalities still lack specificity in that they do not distinguish latent tuberculous infection from active disease and may be influenced by immunosuppressive states [3–5].
Culture is still the gold standard for the diagnosis of tuberculosis; however, it may not be readily available and results may take several weeks . Nucleic acid amplification tests, specifically polymerase chain reaction, have emerged as a powerful tool for detection of the mycobacterial genome in clinical and research specimens and indicate high specificity and variable sensitivity, depending on the clinical setting and tested specimens [3, 6]. In patients with positive results, quantitative PCR analysis of ocular samples may be useful but still cannot exclude the diagnosis in individuals with negative results because of variable sensitivity and should still take into consideration the entire clinical picture [3, 7, 8].
Chorioretinal biopsy allowed PCR and special tissue stains which helped guide management in our patient. Given the biopsy results showing noncaseating granulomas in the absence of mycobacterial infection, the patient was diagnosed with and treated for presumed ocular sarcoidosis. A definitive diagnosis of sarcoidosis requires a biopsy showing noncaseating granulomas in the absence of mycobacterial infection. This patient tested positive for QuantiFERON-TB Gold, suggesting mycobacteria as the etiology for the multifocal choroiditis. However, tuberculostatic drugs are associated with significant side effects , and therefore, caution should be used when prescribing these medications. Our patient’s intolerance to antituberculosis medications prompted further evaluations including a chorioretinal biopsy which led to the diagnosis of presumed ocular sarcoidosis.
Ocular lesions in sarcoidosis occur in 20% to 30% of patients and are most serious because of the threat of blindness . A variety of clinical patterns of inflammatory disease have been described including isolated anterior uveitis, intermediate uveitis, panuveitis, peripheral retinal vasculitis, isolated or multifocal chorioretinal or retinal pigment epithelial lesions and optic nerve granulomata, or occasionally scleritis. Peripheral multifocal choroiditis occurs mainly in women 55 years of age and is characterized by peripheral punched-out lesions associated with intraocular inflammation. Posterior uveitis may be sight threatening when associated with inflammation of the posterior pole and with cystoid macular edema . Acute anterior uveitis resolves spontaneously or after local therapy with corticosteroids, whereas posterior uveitis oftentimes requires systemic treatment. The key is timely diagnosis in order to promptly treat ocular inflammation.
Multifocal chorioretinal lesions of unknown etiology can be a diagnostic, and thus therapeutic, dilemma for the physician. Although a laboratory work-up is useful in the diagnosis, it may not always lead to the accurate diagnosis. Although more invasive procedures such as chorioretinal biopsy carry the potential for serious complications, the results may assist in the proper diagnosis and guide management in difficult cases.
The research described in this report is in compliance with the Helsinki Declaration. Written informed consent was obtained from the patient for publication of this report and accompanying images.
- Wroblewski KJ, Hidayat AA, Neafie RC, Rao NA, Zapor M: Ocular tuberculosis: a clinicopathologic and molecular study. Ophthalmology 2011,118(4):772–777. 10.1016/j.ophtha.2010.08.011PubMedView Article
- Rosenbaum JT, Wernick R: The utility of routine screening of patients with uveitis for systemic lupus erythematosus or tuberculosis. A Bayesian analysis. Arch Ophthalmol 1990,108(9):1291–1293. 10.1001/archopht.1990.01070110107034PubMedView Article
- Dinnes J, Deeks J, Kunst H, Gibson A, Cummins E, Waugh N, Drobniewski F, Lalvani A: A systemic review of rapid diagnostic tests for the detection of tuberculosis infection. Health Technol Assess 2007,11(3):1–196.PubMed
- Albini TA, Karakousis PC, Rao NA: Interferon-gamma release assays in the diagnosis of tuberculous uveitis. Am J Ophthalmol 2008,146(4):486–488. 10.1016/j.ajo.2008.06.021PubMedView Article
- Pai M, Zwerling A, Menzies D: Systematic review: T-cell-based assays for the diagnosis of latent tuberculosis infection: an update. Ann Intern Med 2008,149(3):177–184.PubMed CentralPubMedView Article
- Parashar D, Chauhan DS, Sharma VD, Katoch VM: Applications of real-time PCR technology to mycobacterial research. Indian J Med Res 2006,124(4):385–398.PubMed
- Tan SH, Tan BH, Goh CL, Tan KC, Tan MF, Ng WC, Tan WC: Detection of Mycobacterium tuberculosis DNA using polymerase chain reaction in cutaneous tuberculosis and tuberculids. Int J Dermatol 1999, 38: 122–127. 10.1046/j.1365-4362.1999.00576.xPubMedView Article
- Vasconelos-Santos DV, Zierhut M, Rao NA: Strengths and weaknesses of diagnostic tools to tuberculous uveitis. Ocul Immunol Inflamm 2009, 17: 351–355. 10.3109/09273940903168688View Article
- Forget EJ, Menzies D: Adverse reactions to first-line antituberculosis drugs. Expert Opin Drug Saf 2006,5(2):231–249. 10.1517/14740322.214.171.124PubMedView Article
- James DG, Neville E, Langley DA: Ocular sarcoidosis. Trans Ophthalmol Soc UK 1976, 96: 133.PubMed
- Lardenoye CW, van der Lelij A, de Loos WS, Treffers WF, Rothova A: Peripheral multifocal chorioretinitis: a distinct clinical entity? Ophthalmology 1997, 104: 1820–1826.PubMedView Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.