Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience

Ferrer, Consuelo; Alió, Jorge L.

doi:10.1007/s12348-011-0019-9

Journal of Ophthalmic Inflammation and Infection

Table 2 Fungal DNA detection and identification to diagnose mycotic keratitis

From: Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience

First author	Year	No. of eyes	Target DNA	Detection	Molecular identification	Level of molecular identification
Alexandrakis16	1998	3	Cutinase	PCR	Specific PCR	Fusarium solani
Ferrer8	2001	11	ITSs-5.8S rRNA	Nested PCR	DNA sequencing	Species level
Gaudio14	2002	30	18S rRNA	Nested PCR	Nested PCR	C. albicans, A. fumigatus, F. oxysporum
Ferrer21	2002	1	ITSs-5.8S rRNA	PCR	DNA sequencing	Alternaria alternata
Rishi22	2003	1	ITSs-5.8S rRNA	PCR	Not done	Not done
Guarro23	2003	1	ITSs-5.8S rRNA	Culture	DNA sequencing	Fusarium polyphialidicum
Ferrer24	2003	1	ITSs-5.8S rRNA	PCR	DNA sequencing	Alternaria infectoria
Mancini25	2005	1	ITSs-5.8S rRNA	PCR	DNA sequencing	Scedosporium apiospermum
Kumar17	2005	4	ITSs-5.8S rRNA	PCR	SSCP	Genus level
Kumar18	2005	4	ITSs-5.8S rRNA	PCR	SSCP	Genus level
Kumar19	2006	4	28S rRNA	PCR	SSCP	Genus level
Ferrer9	2006	1	ITSs-5.8S rRNA	PCR	DNA sequencing	Candida parapsilosis
Donnio26	2006	1	ITSs-5.8S rRNA	PCR	DNA sequencing	Lasiodiplodia theobromae
Ghosh27	2007	32	ITSs-5.8S rRNA	PCR	DNA sequencing	Species level
Suzuki28	2007	1	ITSs-5.8S rRNA	PCR	DNA sequencing	Malasezia restricta
Yoon29	2008	1	18S rRNA	PCR	DNA sequencing	Scedosporium apiospermum
Laich30	2008	1	ITSs-5.8S and 28S rRNA	PCR	DNA sequencing	Alternaria alternata
Kim31	2008	108	18S rRNA	PCR	DNA sequencing	Species level
Embong32	2008	30	18S rRNA	PCR	DNA sequencing	Species level
Bagyalakshmi33	2008	12	ITSs-5.8S rRNA	PCR	DNA sequencing	Botryosphaeria Lasiodiplodia ssp., Thielavia tortuosa, Glomerulla singulata, Macrophomina phaseolina, Rhizoctonia bataticola, and Podospora spp.
Vengayil12	2009	40	28S rRNA	PCR	not done	Not done
Dong34	2009	42	ITSs-5.8S rRNA	Microarray	Fluorescent beads	F. solani, F. moniliforme, F. oxysporum, A. fumigatus, A. flavus
Badiee35	2009	38	18S rRNA	Nested PCR	Nested PCR	C. albicans, A. fumigatus, F. solani
Ferrer10, 11	2010	1	ITSs-5.8S rRNA	PCR	DNA sequencing	Pyrenochaeta keratinophila
Itahashi36	2010	40	ITS2	Real-time PCR	Real-time PCR	Candida spp.
Menassa 37	2010	15	ITSs-5.8S rRNA	Real-time PCR	Real-time PCR	C. albicans, Aspergillus spp., Fusarium spp.

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